Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase
نویسندگان
چکیده
منابع مشابه
On the catalytic mechanism of human ATP citrate lyase.
ATP citrate lyase (ACL) catalyzes an ATP-dependent biosynthetic reaction which produces acetyl-coenzyme A and oxaloacetate from citrate and coenzyme A (CoA). Studies were performed with recombinant human ACL to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction and the identity of the active site residues involved. Inactivation of ACL by treatment with diethyl...
متن کاملACLY (ATP citrate lyase)
Other names: ACL, ATPCL, CLATP HGNC (Hugo): ACLY Location: 17q21.2 Note Note that the International Union for Biochemistry and Molecular Biology (IUBMB)'s enzyme nomenclature accepts ATP citrate synthase as the name for ACLY's encoded protein (EC 2.3.3.8). However, ATP citrate lyase is more commonly used and other names include citrate cleavage enzyme, ATP-citrate (pro-S)-lyase, ATPCL, CLATP. A...
متن کاملATP-citrate lyase deficiency in the mouse.
ATP-citrate lyase (Acly) is one of two cytosolic enzymes that synthesize acetyl-coenzyme A (CoA). Because acetyl-CoA is an essential building block for cholesterol and triglycerides, Acly has been considered a therapeutic target for hyperlipidemias and obesity. To define the phenotype of Acly-deficient mice, we created Acly knockout mice in which a beta-galactosidase marker is expressed from Ac...
متن کاملEffect of vanadium ions on ATP citrate lyase.
We have shown previously that vanadium ions (vanadate and vanadyl) inhibit autophosphorylation of histidine but not that of serine in ATP citrate lyase (ACL). Here we report the results concerning the effect of monovanadate (+ oligomers), decavanadate as well as vanadyl on the activity of ACL of the rat liver. Susceptibility of ACL to inhibition by vanadate was rather low. Vanadate at concentra...
متن کاملA novel direct homogeneous assay for ATP citrate lyase.
ATP citrate lyase (ACL) is a cytosolic enzyme that catalyzes the synthesis of acetyl-CoA and oxaloacetate using citrate, CoA, and ATP as substrates and Mg(2+) as a necessary cofactor. The ACL-dependent synthesis of acetyl-CoA is thought to be an essential step for the de novo synthesis of fatty acids and cholesterol. For this reason, inhibition of ACL has been pursued as a strategy to treat dys...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2002
ISSN: 0014-2956
DOI: 10.1046/j.1432-1033.2002.03016.x